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After boiling, 450 uL of ice cold 1X Lysis buffer (1X RIPA, supplemented with 1X protease inhibitor, 1X phosphatase inhibitor, 50 mM N-Ethylmalamide) was then added to the sample, and cells were left on ice for 20 min with vortexing every 5 min for 10 s

The sheath around the nerve that provides it with blood supply and nutrients is compressed, disrupting the blood flow

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